Culture collection

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Do not disinfect the surface.
Use sterile scissors to clip hair.
Use a sterile swab to collect a sample by rolling it across the border of the collarette a few times.
Swab can be moisten to increase bacterial yield from epidermial collarettes ²⁶
Place the collected sample in a transport medium or sterile container.

It can be possible to squeeze a small amount of inflammatory fluid from the papule.
Otherwise, collect a sample by performing a biopsy of the papule.
Provide local anesthesia.
Clip hair with sterile scissors or clippers.
Clean the skin surface with a single wipe of 70% alcohol and allow the alcohol to dry prior to sampling.
Use a sterile punch and surgical instruments to collect a sample of the papule.
Place tissue sample in a sterile container or transport medium.
Alternatively, a sample of the papule can be collected with a sterile needle inserted in the tissue and collecting the expressed blood or exudate. It is recommended to disinfect the papule as described prior to collecting the sample.